• Overview
• Overview for teachers
• The mechanism of PCR
• Denaturation
• Annealing
• Continuing the cycle
• PCR and agriculture
• PCR practical
• Sample assessment (PDF)
• Glossary
• Case study 1 - foreign fish in our market
• Learning outcomes for students
• PD for teachers
• Teacher information
• Experiment 1 - performing a BLAST search (PDF)
• Experiment 2 - creating a phylogenetic tree (PDF)
• Laboratory activity - Oxidation of Fish Oil (PDF)
• Case study 2 - oyster bloom
• Learning outcomes for students
• PD for teachers
• Teacher information
• The oyster industry
• Background of oysters
• Aquaculture in South Australia
• Experiment 1 - induction of spawning in oysters (PDF)
• Experiment 2 - observing the male and female oyster gamates (PDF)

Learning outcomes for students

1. To learn the technique of DNA extraction from commercially available fish species in Australia using a DNA extraction kit.
2. To learn the technique of the Polymerase Chain Reaction (PCR).
3. To separate the amplified DNA by agrose gel electrophoresis.
4. To differentiate species of fish using the results from DNA sequencing using BLAST searches.